Morphometric features of rat pinealocytes in conditions of chronic ethanol intoxication

Ethanol has chronobiological effects, which are associated with inhibition of melatonin synthesis and secretion disruption the sleep-wake cycle. is known to cause sleep fragmentation due frequent awakenings, prolong wakefulness, reduce duration slow phase. At same time, changes in morphology pineal gland under chronic exposure ethanol remain poorly studied. Of particular interest basic morphometric parameters pinealocytes, because they a marker functional state gland. The aim study was morphometrically features morphological rat pinealocytes physiological norm intoxication. To achieve this goal, we used 20 laboratory male rats: control group an experimental group. standard vivarium conditions. For group, alcohol intoxication modeled by injecting 40% solution at rate 12 mg/kg body weight intragastric 4 times day. were studied on day 30 from start study. According results measurements, significant increase light decrease dark cells established. It determined that average values cytoplasm area 54.55% (p<0.05), nucleus increases 61.32% nucleolus 32.84% (p<0.05) compared decreases 27.2% 37.33% (p<0.05). Changes ratio also noted. An number active 8.17% found. detected indicate high activity cells, compensatory nature response apoptosis pinealocytes.